Hotspot

ycf1

SSC · 122325-127943 · 5619 bp

Back to Moehringia

Pi 0.00285

MarkerSeek score 48.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5619
variable_sites: 16
pi: 0.00285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTGTCGGTATGTCGTCTTCA	TCCAGAAGAAGGACAAATTGGT	50.0	40.9	57.1	57.5	206-212 bp	1.0	54.6
ycf1_p2	TGTCGGTATGTCGTCTTCAG	TCCAGAAGAAGGACAAATTGGT	50.0	40.9	57.1	57.5	205-211 bp	1.0	54.6
ycf1_p3	GTCGGTATGTCGTCTTCAGT	TCCAGAAGAAGGACAAATTGGT	50.0	40.9	57.1	57.5	204-210 bp	1.0	54.6
ycf1_p4	TGATCTGTCGGTATGTCGTC	TCCAGAAGAAGGACAAATTGGT	50.0	40.9	56.9	57.5	210-216 bp	1.0	52.8
ycf1_p5	TCTGTCGGTATGTCGTCTTC	TCCAGAAGAAGGACAAATTGGT	50.0	40.9	56.8	57.5	207-213 bp	1.0	52.3