Hotspot

rps2

LSC · 14621-15331 · 711 bp

Back to Moehringia

Pi 0

MarkerSeek score 26.10

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GGAAACCCCCTATTTGCCAA	ATAAACCGCAGCATTCCCTT	50.0	45.0	58.1	57.9	1444-1453 bp	1.0	79.2
rps2_p2	TTGGAAACCCCCTATTTGCC	ATAAACCGCAGCATTCCCTT	50.0	45.0	58.1	57.9	1446-1455 bp	1.0	79.2
rps2_p3	GGAAACCCCCTATTTGCCAA	GGGTTGTGTATCAATGGCCA	50.0	50.0	58.1	58.2	1169-1171 bp	1.0	78.9
rps2_p4	GGAAACCCCCTATTTGCCAA	TGGGTTGTGTATCAATGGCC	50.0	50.0	58.1	58.2	1170-1172 bp	1.0	78.9
rps2_p5	TTGGAAACCCCCTATTTGCC	GGGTTGTGTATCAATGGCCA	50.0	50.0	58.1	58.2	1171-1173 bp	1.0	78.9