Hotspot

ndhK

LSC · 48238-48921 · 684 bp

Back to Moehringia

Pi 0.00292

MarkerSeek score 60.40

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 684
variable_sites: 2
pi: 0.00292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	AGGTTGACTTTACGATACGATGA	CATGGCGAAAAGGAGCATTG	39.1	50.0	57.4	58.1	874 bp	1.0	50.9
ndhK_p2	GGTTGACTTTACGATACGATGA	CATGGCGAAAAGGAGCATTG	40.9	50.0	56.0	58.1	873 bp	1.0	47.8
ndhK_p3	AGGTTGACTTTACGATACGATG	CATGGCGAAAAGGAGCATTG	40.9	50.0	56.0	58.1	874 bp	1.0	47.8
ndhK_p4	AGGTTGACTTTACGATACGATGAA	CATGGCGAAAAGGAGCATTG	37.5	50.0	58.0	58.1	874 bp	1.0	47.2
ndhK_p5	AGGTTGACTTTACGATACGA	CATGGCGAAAAGGAGCATTG	40.0	50.0	53.9	58.1	874 bp	1.0	46.5