Hotspot

matK

LSC · 1691-3202 · 1512 bp

Back to Moehringia

Pi 0.00265

MarkerSeek score 60.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 4
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CCACACATTTAGGTAGAATTTCCA	TGATAACTCAAGCAACCCCC	37.5	50.0	56.9	57.5	1620 bp	1.0	40.2
matK_p2	TCCACACATTTAGGTAGAATTTCCA	TGATAACTCAAGCAACCCCC	36.0	50.0	58.2	57.5	1621 bp	1.0	40.2
matK_p3	TCCACACATTTAGGTAGAATTTCC	TGATAACTCAAGCAACCCCC	37.5	50.0	56.7	57.5	1621 bp	1.0	40.2
matK_p4	CCACACATTTAGGTAGAATTTCCA	GATAACTCAAGCAACCCCCT	37.5	50.0	56.9	57.2	1619 bp	1.0	40.2
matK_p5	TCCACACATTTAGGTAGAATTTCCA	GATAACTCAAGCAACCCCCT	36.0	50.0	58.2	57.2	1620 bp	1.0	40.2