Hotspot

ndhD-psaC

SSC · 115557-115691 · 135 bp

Back to Moehringia

Pi 0.0296

MarkerSeek score 63.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 135
variable_sites: 4
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAACCTGCAAATATCGGCA	GAACGGAAGACTGTGTTGGT	45.0	50.0	57.6	58.1	308 bp	1.0	77.3
ndhD-psaC_p2	AGAACCTGCAAATATCGGCA	AGAACGGAAGACTGTGTTGG	45.0	50.0	57.6	57.8	309 bp	1.0	75.8
ndhD-psaC_p3	AGAACCTGCAAATATCGGCA	CCAAGAACGGAAGACTGTGT	45.0	50.0	57.6	57.8	312 bp	1.0	75.8
ndhD-psaC_p4	AGAACCTGCAAATATCGGCA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.6	57.7	285 bp	1.0	75.2
ndhD-psaC_p5	AGAACCTGCAAATATCGGCA	CGGAAGACTGTGTTGGTTGT	45.0	50.0	57.6	58.3	305 bp	1.0	75.0