Hotspot

psbE-petL

LSC · 64071-65102 · 1032 bp

Back to Moehringia

Pi 0.00291

MarkerSeek score 60.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 3
pi: 0.00291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	1148-1149 bp	1.0	69.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCT	50.0	50.0	57.8	56.8	1147-1148 bp	1.0	69.1
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACCT	50.0	47.6	57.8	58.3	1148-1149 bp	1.0	68.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGCTCAGACC	55.0	50.0	58.2	56.8	1143-1144 bp	1.0	68.7
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCT	55.0	50.0	58.2	56.8	1142-1143 bp	1.0	68.7