Hotspot

petA-psbJ

LSC · 62374-63270 · 897 bp

Back to Moehringia

Pi 0.00446

MarkerSeek score 61.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 4
pi: 0.00446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTACGCGTCCAAGGTCTTTT	GGTTGGGCTCATCGCTATAG	45.0	55.0	57.8	57.9	1030-1031 bp	1.0	77.8
petA-psbJ_p2	CATTACGCGTCCAAGGTCTT	GGTTGGGCTCATCGCTATAG	50.0	55.0	58.3	57.9	1032-1033 bp	1.0	77.5
petA-psbJ_p3	GCGTCCAAGGTCTTTTGTTC	GGTTGGGCTCATCGCTATAG	50.0	55.0	57.6	57.9	1026-1027 bp	1.0	76.5
petA-psbJ_p4	ATTACGCGTCCAAGGTCTTT	GGTTGGGCTCATCGCTATAG	45.0	55.0	57.5	57.9	1031-1032 bp	1.0	76.0
petA-psbJ_p5	TACGCGTCCAAGGTCTTTTG	GGTTGGGCTCATCGCTATAG	50.0	55.0	58.5	57.9	1029-1030 bp	1.0	75.7