Hotspot

psaA-ycf3

LSC · 40195-41108 · 914 bp

Back to Moehringia

Pi 0.0011

MarkerSeek score 60.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCTCAAACGACGTTTTGACG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	1076-1085 bp	1.0	80.4
psaA-ycf3_p2	CTCAAACGACGTTTTGACGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	1075-1084 bp	1.0	80.4
psaA-ycf3_p3	CCTCAAACGACGTTTTGACG	GCTATAACGCTTACTCCGGG	50.0	55.0	58.0	57.9	1050-1059 bp	1.0	80.3
psaA-ycf3_p4	CTCAAACGACGTTTTGACGG	GCTATAACGCTTACTCCGGG	50.0	55.0	58.0	57.9	1049-1058 bp	1.0	80.3
psaA-ycf3_p5	GTTCTTGAAAAATGGCCGGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1107-1116 bp	1.0	79.8