Hotspot

rps2-rpoC2

LSC · 15332-15597 · 266 bp

Back to Moehringia

Pi 0.0193

MarkerSeek score 66.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 266
variable_sites: 5
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GGTGACATTCGAGGATTCCA	TATTCGGGGGAGAAATGGGA	50.0	50.0	57.3	57.8	455-462 bp	1.0	75.0
rps2-rpoC2_p2	TGACCAAAATGAACTCCCGC	TATTCGGGGGAGAAATGGGA	50.0	50.0	58.8	57.8	422-429 bp	1.0	74.6
rps2-rpoC2_p3	GGTGACATTCGAGGATTCCA	CGGGGGAGAAATGGGAGATA	50.0	55.0	57.3	58.3	451-458 bp	1.0	74.4
rps2-rpoC2_p4	TGACCAAAATGAACTCCCGC	CGGGGGAGAAATGGGAGATA	50.0	55.0	58.8	58.3	418-425 bp	1.0	74.1
rps2-rpoC2_p5	TTCGAGTACCGTGACCAAAA	TATTCGGGGGAGAAATGGGA	45.0	50.0	57.1	57.8	433-440 bp	1.0	73.5