Hotspot

rpl16

LSC · 83222-84657 · 1436 bp

Back to Mitella

Pi 0.0191

MarkerSeek score 56.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1436
variable_sites: 27
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	ATATCCTCTGGTGTCGAGGG	50.0	55.0	58.0	58.0	2525-2537 bp	1.0	82.1
rpl16_p2	CGCACCAAAAACTCGAGTTC	CTCTGGTGTCGAGGGAATTG	50.0	55.0	58.0	58.0	2520-2532 bp	1.0	82.0
rpl16_p3	AACTGTCTCAATTCTCGGGC	ATATCCTCTGGTGTCGAGGG	50.0	55.0	57.9	58.0	2548-2560 bp	1.0	81.4
rpl16_p4	AACTGTCTCAATTCTCGGGC	CTCTGGTGTCGAGGGAATTG	50.0	55.0	57.9	58.0	2543-2555 bp	1.0	81.3
rpl16_p5	ATCGCACCAAAAACTCGAGT	ATATCCTCTGGTGTCGAGGG	45.0	55.0	58.1	58.0	2527-2539 bp	1.0	81.3