Hotspot

rpl22-rps19

LSC · 85972-86025 · 54 bp

Back to Mitella

Pi 0.037

MarkerSeek score 73.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 54
variable_sites: 2
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGATCAACTACTCTTCGCGC	TAAATTTCCGGGGACATGCG	50.0	50.0	58.1	58.6	187 bp	1.0	75.2
rpl22-rps19_p2	AACTACTCTTCGCGCTTTGT	TAAATTTCCGGGGACATGCG	45.0	50.0	57.8	58.6	182 bp	1.0	74.8
rpl22-rps19_p3	GATCAACTACTCTTCGCGCT	TAAATTTCCGGGGACATGCG	50.0	50.0	57.8	58.6	186 bp	1.0	74.4
rpl22-rps19_p4	TACTCTTCGCGCTTTGTGAG	TAAATTTCCGGGGACATGCG	50.0	50.0	58.3	58.6	179 bp	1.0	73.6
rpl22-rps19_p5	GGAACGTCCACGAATCTGAT	TAAATTTCCGGGGACATGCG	50.0	50.0	57.7	58.6	203 bp	1.0	73.5