Hotspot

rpl14-rpl16

LSC · 83082-83221 · 140 bp

Back to Mitella

Pi 0.0299

MarkerSeek score 65.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 4
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TACACATCAATTCTCGGGCC	AGCAGCATCCAAAATGCCTA	50.0	45.0	57.7	57.9	244-250 bp	1.0	79.4
rpl14-rpl16_p2	TACACATCAATTCTCGGGCC	TAGCAGCATCCAAAATGCCT	50.0	45.0	57.7	57.9	245-251 bp	1.0	79.4
rpl14-rpl16_p3	GCTGTTGTCCGCTACATTCA	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.6	57.9	222-228 bp	1.0	77.9
rpl14-rpl16_p4	GCTGTTGTCCGCTACATTCA	TAGCAGCATCCAAAATGCCT	50.0	45.0	58.6	57.9	223-229 bp	1.0	77.9
rpl14-rpl16_p5	TACACATCAATTCTCGGGCC	TATGAAATGGGCGGAGTAGC	50.0	50.0	57.7	57.5	294-300 bp	1.0	75.7