Hotspot

rpl20-rps12

LSC · 71049-71823 · 775 bp

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Pi 0.0469
MarkerSeek score 73.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
775
variable_sites
36
pi
0.0469
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CCCGAAAGCTTGATGCAAAT CGAGGAACATGTACTAGGGTG 45.0 52.4 57.3 57.6 875-883 bp 1.0 67.2
rpl20-rps12_p2 TAAGTCTTGAATGAGCCCCC CGAGGAACATGTACTAGGGTG 50.0 52.4 56.9 57.6 893-901 bp 1.0 63.6
rpl20-rps12_p3 CCCGAAAGCTTGATGCAAAT CGAGGAACATGTACTAGGGT 45.0 50.0 57.3 56.1 875-883 bp 1.0 63.3
rpl20-rps12_p4 GTAAGTCTTGAATGAGCCCCC CGAGGAACATGTACTAGGGTG 52.4 52.4 58.4 57.6 894-902 bp 1.0 61.6
rpl20-rps12_p5 CCCCGAAAGCTTGATGCAAA CGAGGAACATGTACTAGGGTG 50.0 52.4 59.4 57.6 876-884 bp 1.0 61.3