Hotspot

rbcL-accD

LSC · 58678-59270 · 593 bp

Back to Mitella

Pi 0.0135

MarkerSeek score 65.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 8
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	CAACATCGAATTGAACCGCC	55.0	50.0	57.7	58.1	750 bp	1.0	77.7
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	TGAACCGCCGTTTTTCCATA	55.0	45.0	57.7	58.1	739 bp	1.0	77.7
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	AATTGAACCGCCGTTTTTCC	55.0	45.0	57.7	57.9	742 bp	1.0	77.4
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	AACCGCCGTTTTTCCATAGA	55.0	45.0	57.7	57.8	737 bp	1.0	77.0
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	CGAATTGAACCGCCGTTTTT	55.0	45.0	57.7	58.2	744 bp	1.0	76.6