Hotspot

petN-psbM

LSC · 29812-30504 · 693 bp

Back to Mitella

Pi 0.0231

MarkerSeek score 72.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 16
pi: 0.0231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	855-863 bp	1.0	69.1
petN-psbM_p2	GGGGACACAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	877-885 bp	1.0	68.6
petN-psbM_p3	AGGGGACACAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	878-886 bp	1.0	68.5
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	794-802 bp	1.0	67.6
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	855-863 bp	1.0	64.9