Hotspot

atpH-atpI

LSC · 14340-15464 · 1125 bp

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Pi 0.0235

MarkerSeek score 66.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1125
variable_sites: 26
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1202-1212 bp	1.0	81.5
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1194-1204 bp	1.0	81.3
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1258-1268 bp	1.0	80.8
atpH-atpI_p4	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1195-1205 bp	1.0	80.5
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1203-1213 bp	1.0	80.3