Hotspot

rps16-trnQ-UUG

LSC · 6229-7579 · 1351 bp

Back to Mitella

Pi 0.0249

MarkerSeek score 75.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1351
variable_sites: 33
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1424-1692 bp	1.0	81.4
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1435-1703 bp	1.0	80.8
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1445-1713 bp	1.0	79.1
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1446-1714 bp	1.0	79.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AGAAAGCTAAATGGGGCGTG	45.0	50.0	58.1	58.5	1483-1751 bp	1.0	77.6