Hotspot

rpl23

LSC · 59304-59576 · 273 bp

Back to Miscanthus

Pi 0.00122

MarkerSeek score 26.90

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: LSC
length_bp: 273
variable_sites: 1
pi: 0.00122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	TGCAAATGGAGTGCTGAACT	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	1102 bp	1.0	79.7
rpl23_p2	GGCTTCAGGGGGTATTCATG	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.0	57.9	1316 bp	1.0	79.6
rpl23_p3	GTTATACCGGTGGCTTCAGG	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.1	57.9	1327 bp	1.0	79.3
rpl23_p4	GCCTGTGTACAAGCTCGTAA	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.9	57.9	1171 bp	1.0	79.2
rpl23_p5	CACTCAGGACTGGGTATCCA	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.1	57.9	1355 bp	1.0	78.7