Hotspot

psaB

LSC · 39738-41942 · 2205 bp

Back to Miscanthus

Pi 0.000151

MarkerSeek score 44.90

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 1
pi: 0.000151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	CTAAAAAGGCGGATCCTCCC	TACAAGGACGTGCTGTAGGA	55.0	50.0	58.0	58.1	2401 bp	1.0	79.1
psaB_p2	GGATCCTCCCTCTCCATCAA	TACAAGGACGTGCTGTAGGA	55.0	50.0	57.9	58.1	2391 bp	1.0	78.5
psaB_p3	CTAAAAAGGCGGATCCTCCC	AAGGACGTGCTGTAGGAGTA	55.0	50.0	58.0	57.8	2398 bp	1.0	78.1
psaB_p4	CTAAAAAGGCGGATCCTCCC	AGGACGTGCTGTAGGAGTAA	55.0	50.0	58.0	57.8	2397 bp	1.0	78.1
psaB_p5	CCTCCCTCTCCATCAAGAGT	TACAAGGACGTGCTGTAGGA	55.0	50.0	57.8	58.1	2387 bp	1.0	78.0