Hotspot

rpl32-trnL-UAG

SSC · 109250-109772 · 523 bp

Back to Miscanthus

Pi 0.00535

MarkerSeek score 38.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 523
variable_sites: 6
pi: 0.00535
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.4	58.0	619-620 bp	1.0	77.4
rpl32-trnL-UAG_p2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.4	58.1	622-623 bp	1.0	77.0
rpl32-trnL-UAG_p3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	592-593 bp	1.0	76.5
rpl32-trnL-UAG_p4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.4	57.8	585-586 bp	1.0	76.0
rpl32-trnL-UAG_p5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.4	58.2	606-607 bp	1.0	76.0