Hotspot

ndhC-trnV-UAC

LSC · 52844-53790 · 947 bp

Back to Miscanthus

Pi 0.00127
MarkerSeek score 44.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
947
variable_sites
3
pi
0.00127
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 1073 bp 1.0 79.3
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.0 57.9 1166 bp 1.0 79.1
ndhC-trnV-UAC_p3 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 1074 bp 1.0 77.5
ndhC-trnV-UAC_p4 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 1072 bp 1.0 76.9
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT AGCCCAATGCAGAAAAATGC 50.0 45.0 58.3 57.9 1165 bp 1.0 76.7