Hotspot

psaA-ycf3

LSC · 44221-44824 · 604 bp

Back to Miscanthus

Pi 0.00188

MarkerSeek score 44.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.0	57.7	835-836 bp	1.0	77.9
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	800-801 bp	1.0	77.4
psaA-ycf3_p3	GGGGCCCTTAGCTATTGTTC	GCTATAGCGCTTACTCCAGG	55.0	55.0	58.0	57.6	802-803 bp	1.0	77.2
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.1	57.6	767-768 bp	1.0	76.7
psaA-ycf3_p5	GGTTCCGACGGACGAATAAT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	738-739 bp	1.0	76.3