Hotspot

rpoB-rpoC1

LSC · 25220-25256 · 37 bp

Back to Miscanthus

Pi 0.00901

MarkerSeek score 44.30

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 37
variable_sites: 1
pi: 0.00901
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	TCTGAGAAGAACTTCCGGGT	CCAACGACTTCCCCATTAGG	50.0	55.0	58.0	58.0	180 bp	1.0	80.0
rpoB-rpoC1_p2	TCCGCCAGAATCTTTTCGAG	CCAACGACTTCCCCATTAGG	50.0	55.0	58.0	58.0	253 bp	1.0	79.9
rpoB-rpoC1_p3	TCTGAGAAGAACTTCCGGGT	TTGTTGAGGGGAAACGAGTC	50.0	50.0	58.0	57.8	133 bp	1.0	78.5
rpoB-rpoC1_p4	TCCGCCAGAATCTTTTCGAG	TTGTTGAGGGGAAACGAGTC	50.0	50.0	58.0	57.8	206 bp	1.0	78.3
rpoB-rpoC1_p5	CCGCCAGAATCTTTTCGAGT	CCAACGACTTCCCCATTAGG	50.0	55.0	58.3	58.0	252 bp	1.0	77.8