Hotspot

trnC-GCA-rpoB

LSC · 20783-21991 · 1209 bp

Back to Miscanthus

Pi 0.00551

MarkerSeek score 39.70

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1209
variable_sites: 14
pi: 0.00551
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1356-1373 bp	1.0	79.3
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1354-1371 bp	1.0	79.3
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1357-1374 bp	1.0	79.3
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCGCCAAGCCTTGATTAA	50.0	45.0	58.1	57.8	1344-1361 bp	1.0	79.0
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CTTCCGCCAAGCCTTGATTA	50.0	50.0	58.1	58.2	1345-1362 bp	1.0	78.7