Hotspot

ycf1

SSC · 124812-130412 · 5601 bp

Back to Mirabilis

Pi 0.0127

MarkerSeek score 45.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 71
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGAGTGGGCGATTTGTGAA	GCGTGTTCAAAGACGGAAAA	45.0	45.0	58.0	57.5	991 bp	0.5	56.1
ycf1_p2	TTTGAGTGGGCGATTTGTGA	GCGTGTTCAAAGACGGAAAA	45.0	45.0	58.0	57.5	992 bp	0.5	56.1
ycf1_p3	GTTTGAGTGGGCGATTTGTG	GCGTGTTCAAAGACGGAAAA	50.0	45.0	57.9	57.5	993 bp	0.5	55.9
ycf1_p4	TTGAGTGGGCGATTTGTGAA	TGCGTGTTCAAAGACGGAAA	45.0	45.0	58.0	58.6	992 bp	0.5	54.8
ycf1_p5	TTTGAGTGGGCGATTTGTGA	TGCGTGTTCAAAGACGGAAA	45.0	45.0	58.0	58.6	993 bp	0.5	54.8