Hotspot

rpoC1

LSC · 20799-23603 · 2805 bp

Back to Mirabilis

Pi 0.00927

MarkerSeek score 47.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2805
variable_sites: 26
pi: 0.00927
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AAGACACGAATTTCACCGGT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.8	57.9	2968-2976 bp	1.0	78.0
rpoC1_p2	AAGACACGAATTTCACCGGT	CGATCTTTGGCTCTGGAACT	45.0	50.0	57.8	57.6	2967-2975 bp	1.0	75.6
rpoC1_p3	AAGACACGAATTTCACCGGT	GCTCGTTCGAGAACTACGAT	45.0	50.0	57.8	57.5	2983-2991 bp	1.0	75.1
rpoC1_p4	AAGACACGAATTTCACCGGT	CTGCTCGTTCGAGAACTACG	45.0	55.0	57.8	58.5	2985-2993 bp	1.0	75.0
rpoC1_p5	ACGAATTTCACCGGTATCGT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.3	57.9	2963 bp	0.5	53.9