Hotspot

rpl22

LSC · 84957-85502 · 546 bp

Back to Mirabilis

Pi 0.0128

MarkerSeek score 55.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 546
variable_sites: 7
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	TGTTTCTAATTCATTAATGGGAGGT	50.0	32.0	58.0	56.5	621 bp	1.0	40.9
rpl22_p2	TGGGTAGTACCAAGTCGGAA	TGTTTCTAATTCATTAATGGGAGGT	50.0	32.0	57.7	56.5	631 bp	1.0	40.9
rpl22_p3	TTGGGTAGTACCAAGTCGGA	TGTTTCTAATTCATTAATGGGAGGT	50.0	32.0	57.7	56.5	632 bp	1.0	40.9
rpl22_p4	ACCAAGTCGGAAACCAAGTG	TGTTTCTAATTCATTAATGGGAGGT	50.0	32.0	58.3	56.5	623 bp	1.0	40.9
rpl22_p5	CAAGTCGGAAACCAAGTGGA	TCATTAATGGGAGGTAAACTT	50.0	33.3	58.0	52.1	611 bp	1.0	40.9