Hotspot

petD

LSC · 78046-79312 · 1267 bp

Back to Mirabilis

Pi 0.0142

MarkerSeek score 55.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1267
variable_sites: 18
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCGCCGGTTGAAGGTAATTT	CATTGCAGCGACTATTCCCT	45.0	50.0	57.8	58.0	1373-1382 bp	1.0	79.3
petD_p2	TCGCCGGTTGAAGGTAATTT	TGCAGCGACTATTCCCTAGA	45.0	50.0	57.8	57.9	1370-1379 bp	1.0	79.1
petD_p3	TCGCCGGTTGAAGGTAATTT	GCACATTGCAGCGACTATTC	45.0	50.0	57.8	57.9	1376-1385 bp	1.0	78.8
petD_p4	ATCGCCGGTTGAAGGTAATT	CATTGCAGCGACTATTCCCT	45.0	50.0	57.6	58.0	1374-1383 bp	1.0	77.5
petD_p5	ATCGCCGGTTGAAGGTAATT	TGCAGCGACTATTCCCTAGA	45.0	50.0	57.6	57.9	1371-1380 bp	1.0	77.3