Hotspot

ccsA

SSC · 115331-116320 · 990 bp

Back to Mirabilis

Pi 0.00508

MarkerSeek score 50.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 990
variable_sites: 5
pi: 0.00508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAACAAGCCGCTATGGTGAA	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.1	58.0	1574-1580 bp	1.0	80.7
ccsA_p2	AACAAGCCGCTATGGTGAAA	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.1	58.0	1573-1579 bp	1.0	80.7
ccsA_p3	AAACAAGCCGCTATGGTGAA	GGCATGAGTGGTTTCATTGC	45.0	50.0	58.1	58.0	1767-1773 bp	1.0	80.6
ccsA_p4	AACAAGCCGCTATGGTGAAA	GGCATGAGTGGTTTCATTGC	45.0	50.0	58.1	58.0	1766-1772 bp	1.0	80.6
ccsA_p5	TTCAAACAAGCCGCTATGGT	GGCATGAGTGGTTTCATTGC	45.0	50.0	58.1	58.0	1770-1776 bp	1.0	80.6