Hotspot

rpl32-trnL-UAG

SSC · 114635-115128 · 494 bp

Back to Mirabilis

Pi 0.0291

MarkerSeek score 64.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 494
variable_sites: 13
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAATCTTGAACTTGAAAATGAA	AGCAGCGTGTCTACCAATTT	26.1	45.0	52.8	57.8	506-553 bp	1.0	46.0
rpl32-trnL-UAG_p2	AGAATCTTGAACTTGAAAATGAA	TCCTAAGAGCAGCGTGTCTA	26.1	50.0	52.8	57.9	513-560 bp	1.0	45.9
rpl32-trnL-UAG_p3	AGAATCTTGAACTTGAAAATGAA	GCTCTTGCACTGCTTCCTAA	26.1	50.0	52.8	58.2	527-574 bp	1.0	45.9
rpl32-trnL-UAG_p4	AGAATCTTGAACTTGAAAATGAA	TTGCACTGCTTCCTAAGAGC	26.1	50.0	52.8	58.2	523-570 bp	1.0	45.9
rpl32-trnL-UAG_p5	AGAATCTTGAACTTGAAAATGAA	CTAAGAGCAGCGTGTCTACC	26.1	55.0	52.8	57.8	511-558 bp	1.0	45.9