Hotspot

psbT-psbN

LSC · 75704-75763 · 60 bp

Back to Mirabilis

Pi 0.1

MarkerSeek score 87.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 6
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCTCCGAAAGTTCCGACTAA	GGCAGCCCTCTCAACAATTA	50.0	50.0	57.0	57.9	143 bp	1.0	74.1
psbT-psbN_p2	CCTCCGAAAGTTCCGACTAA	GATCCTTTCGAGGAACACGG	50.0	55.0	57.0	58.4	120 bp	1.0	72.9
psbT-psbN_p3	CCTCCGAAAGTTCCGACTAAA	GGCAGCCCTCTCAACAATTA	47.6	50.0	57.7	57.9	143 bp	1.0	71.9
psbT-psbN_p4	CCTCCGAAAGTTCCGACTAAA	GATCCTTTCGAGGAACACGG	47.6	55.0	57.7	58.4	120 bp	1.0	70.7
psbT-psbN_p5	GCCTCCGAAAGTTCCGACTA	GGCAGCCCTCTCAACAATTA	55.0	50.0	59.5	57.9	144 bp	1.0	70.5