Hotspot

rps4-trnT-UGU

LSC · 46936-47371 · 436 bp

Back to Mirabilis

Pi 0.179

MarkerSeek score 87.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 77
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCCAGGTAAAGCCCCTAGAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	557-559 bp	1.0	86.7
rps4-trnT-UGU_p2	TCCAGGTAAAGCCCCTAGAC	GATGGTCATCGGTTCGACTC	55.0	55.0	58.1	58.2	526-528 bp	1.0	86.5
rps4-trnT-UGU_p3	GACGCGGTTGGTTTCTAAGA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	609-611 bp	1.0	85.9
rps4-trnT-UGU_p4	AAGCCCCTAGACGACGTATT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	549-551 bp	1.0	85.8
rps4-trnT-UGU_p5	AGCCCCTAGACGACGTATTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	548-550 bp	1.0	85.8