Hotspot

rpoC1-rpoB

LSC · 23604-23629 · 26 bp

Back to Mirabilis

Pi 0.0385

MarkerSeek score 68.80

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGGCCCAAGCACTTATTTGT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	58.0	57.9	175 bp	1.0	79.6
rpoC1-rpoB_p2	TGTGGCCCAAGCACTTATTT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	58.0	57.9	177 bp	1.0	79.6
rpoC1-rpoB_p3	TTGTGGCCCAAGCACTTATT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	58.0	57.9	178 bp	1.0	79.6
rpoC1-rpoB_p4	TTTGTGGCCCAAGCACTTAT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	58.0	57.9	179 bp	1.0	79.6
rpoC1-rpoB_p5	TGGCCCAAGCACTTATTTGT	CCCGAGAATGCTCCAGAATC	45.0	55.0	58.0	58.1	219 bp	1.0	79.3