Hotspot

trnS-GCU-trnG-UCC

LSC · 8114-9700 · 1587 bp

Back to Mirabilis

Pi 0.0134

MarkerSeek score 56.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1587
variable_sites: 21
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	CATCGTTAGCTTGGAAGGCT	55.0	50.0	58.6	58.0	1722-1778 bp	1.0	76.3
trnS-GCU-trnG-UCC_p2	CTTTCGTCCACTCAGCCATC	TAGCTTGGAAGGCTAAGGGT	55.0	50.0	58.6	58.0	1716-1772 bp	1.0	76.3
trnS-GCU-trnG-UCC_p3	TTTCGTCCACTCAGCCATCT	CATCGTTAGCTTGGAAGGCT	50.0	50.0	59.0	58.0	1721-1777 bp	1.0	73.2
trnS-GCU-trnG-UCC_p4	TTTCGTCCACTCAGCCATCT	TAGCTTGGAAGGCTAAGGGT	50.0	50.0	59.0	58.0	1715-1771 bp	1.0	73.2
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	CATCGTTAGCTTGGAAGGCT	57.9	50.0	58.1	58.0	1716-1772 bp	1.0	72.7