Hotspot

ycf1

SSC · 129011-134665 · 5655 bp

Back to Mimusops

Pi 0.0039

MarkerSeek score 55.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5655
variable_sites: 22
pi: 0.0039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTACGACCATCGAGGAACT	ACTGATTACCGAGAATTTGAACAG	50.0	37.5	58.2	57.3	292 bp	1.0	41.9
ycf1_p2	TGTACGACCATCGAGGAACT	ACTGATTACCGAGAATTTGAACA	50.0	34.8	58.2	56.2	292 bp	1.0	41.4
ycf1_p3	TGTACGACCATCGAGGAACT	ACCGAGAATTTGAACAGAAA	50.0	35.0	58.2	53.0	285 bp	1.0	41.1
ycf1_p4	TGTACGACCATCGAGGAACT	CTGATTACCGAGAATTTGAACAGA	50.0	37.5	58.2	57.0	291 bp	1.0	41.1
ycf1_p5	TGTACGACCATCGAGGAACT	TGATTACCGAGAATTTGAACAGA	50.0	34.8	58.2	56.0	290 bp	1.0	41.1