Hotspot

trnL-UAA

LSC · 51377-51411 · 35 bp

Back to Mimusops

Pi 0.0286

MarkerSeek score 68.90

Primer available yes

Label trnL-UAA

Feature Metadata

label_name: trnL-UAA
feature_type: tRNA
region: LSC
length_bp: 35
variable_sites: 1
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA_p1	AGGAAACGACATCACAATGAGA	AGGATTTGGCTCAGGATTGC	40.9	50.0	57.7	58.2	177 bp	1.0	60.5
trnL-UAA_p2	AGGAAACGACATCACAATGAGA	GGTTTCCATACCAAGGCTCA	40.9	50.0	57.7	57.5	105 bp	1.0	58.5
trnL-UAA_p3	GGAAACGACATCACAATGAGA	AGGATTTGGCTCAGGATTGC	42.9	50.0	56.3	58.2	176 bp	1.0	56.9
trnL-UAA_p4	AGGAAACGACATCACAATGAG	AGGATTTGGCTCAGGATTGC	42.9	50.0	56.3	58.2	177 bp	1.0	56.8
trnL-UAA_p5	AGGAAACGACATCACAATGAGA	CCATACCAAGGCTCAATCCA	40.9	50.0	57.7	57.3	100 bp	1.0	56.7