Hotspot

ndhF

SSC · 114980-117226 · 2247 bp

Back to Mimusops

Pi 0.00445

MarkerSeek score 47.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 10
pi: 0.00445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	AGGGGGAAGTTCTTGTTTCA	50.0	45.0	58.0	56.6	2944-2950 bp	1.0	68.6
ndhF_p2	AACCGAAGAGATCCGAGTGA	AGGGGGAAGTTCTTGTTTCA	50.0	45.0	58.2	56.6	2846-2852 bp	1.0	67.5
ndhF_p3	ACCGAAGAGATCCGAGTGAA	AGGGGGAAGTTCTTGTTTCA	50.0	45.0	58.2	56.6	2845-2851 bp	1.0	67.5
ndhF_p4	ACGAAGGGGACTAAACAGGA	AAGGGGGAAGTTCTTGTTTCA	50.0	42.9	58.0	57.3	2945-2951 bp	1.0	66.7
ndhF_p5	GGAACAAGAGGGATTCACCG	AGGGGGAAGTTCTTGTTTCA	55.0	45.0	58.3	56.6	2927-2933 bp	1.0	66.7