Hotspot

rpl32-trnL-UAG

SSC · 118434-119337 · 904 bp

Back to Mimusops

Pi 0.00996
MarkerSeek score 53.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
904
variable_sites
9
pi
0.00996
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAAATCTCTTTTGACCGGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 1003-1004 bp 1.0 71.2
rpl32-trnL-UAG_p2 AGGGAAATCTCTTTTGACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 1004-1005 bp 1.0 71.1
rpl32-trnL-UAG_p3 GGGAAATCTCTTTTGACCGGA AGCAGCGTGTCTACCAATTT 47.6 45.0 57.9 57.8 996-997 bp 1.0 70.7
rpl32-trnL-UAG_p4 AGGGAAATCTCTTTTGACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 57.9 57.8 997-998 bp 1.0 70.7
rpl32-trnL-UAG_p5 GGGAAATCTCTTTTGACCGGA CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.9 57.8 1001-1002 bp 1.0 70.6