Hotspot

rpl20-rps12

LSC · 73341-74126 · 786 bp

Back to Mimusops

Pi 0.00763

MarkerSeek score 62.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 6
pi: 0.00763
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	942-946 bp	1.0	75.3
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	941-945 bp	1.0	75.3
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACTTGTAC	50.0	55.0	57.9	58.7	905-909 bp	1.0	74.3
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACTTGTAC	50.0	55.0	57.9	58.7	904-908 bp	1.0	74.3
rpl20-rps12_p5	TCCCCGTCTAATTCTGGTCA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.8	58.5	874-878 bp	1.0	74.3