Hotspot

atpB-rbcL

LSC · 58200-58997 · 798 bp

Back to Mimusops

Pi 0.00127

MarkerSeek score 60.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 1
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	953-959 bp	1.0	77.2
atpB-rbcL_p2	ATTTGAACGATACGCCCCAG	AACACCCGCTTTGAATCCAA	50.0	45.0	58.3	58.3	920-926 bp	1.0	75.8
atpB-rbcL_p3	GATTTGAACGATACGCCCCA	AACACCCGCTTTGAATCCAA	50.0	45.0	58.3	58.3	921-927 bp	1.0	75.7
atpB-rbcL_p4	TGATTTGAACGATACGCCCC	AACACCCGCTTTGAATCCAA	50.0	45.0	58.3	58.3	922-928 bp	1.0	75.7
atpB-rbcL_p5	GGCGGAAAGGCTACATCTAG	TAACACCCGCTTTGAATCCA	55.0	45.0	57.8	57.4	954-960 bp	1.0	75.0