Hotspot

psaA-ycf3

LSC · 45246-46005 · 760 bp

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Pi 0.00133

MarkerSeek score 60.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 1
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	57.9	58.0	950-957 bp	1.0	79.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGCAGAGGCTTGGTTTGAT	50.0	45.0	57.9	58.0	949-956 bp	1.0	79.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	931-938 bp	1.0	79.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	913-920 bp	1.0	79.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	927-934 bp	1.0	77.8