Hotspot

trnC-GCA-petN

LSC · 30019-30785 · 767 bp

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Pi 0.00391
MarkerSeek score 61.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
767
variable_sites
3
pi
0.00391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTAACTA 50.0 50.0 58.4 58.2 841-852 bp 1.0 76.2
trnC-GCA-petN_p2 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTAACTA 50.0 50.0 58.5 58.2 816-827 bp 1.0 75.3
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 902-913 bp 1.0 74.6
trnC-GCA-petN_p4 TATCCTTTTGGCGACATGGC AGCCCAAGCGAGACTAACTA 50.0 50.0 58.6 58.2 874-885 bp 1.0 74.2
trnC-GCA-petN_p5 AGTTCAAATCTGGGTGTCGC AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.5 57.6 877-888 bp 1.0 73.7