Hotspot

psbI-trnS-GCU

LSC · 9631-9772 · 142 bp

Back to Mimusops

Pi 0.00704
MarkerSeek score 45.00
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
142
variable_sites
1
pi
0.00704
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAAGA GGGTTCGAATCCCTCTCTTT 50.0 50.0 57.1 56.9 189 bp 1.0 64.3
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAAGA GGGTTCGAATCCCTCTCTTTC 50.0 52.4 57.1 58.1 189 bp 1.0 64.2
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAAGA GGTTCGAATCCCTCTCTTTCC 50.0 52.4 57.1 58.1 188 bp 1.0 64.2
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGAA GGGTTCGAATCCCTCTCTTT 47.6 50.0 57.8 56.9 189 bp 1.0 62.0
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGAA GGTTCGAATCCCTCTCTTTCC 47.6 52.4 57.8 58.1 188 bp 1.0 61.9