Hotspot

trnK-UUU-rps16

LSC · 4487-5320 · 834 bp

Back to Mimusops

Pi 0.0036

MarkerSeek score 60.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 3
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	977-980 bp	1.0	78.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	976-979 bp	1.0	78.9
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	57.8	58.0	1062-1065 bp	1.0	78.4
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	57.8	58.0	1061-1064 bp	1.0	78.3
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.3	58.0	992-995 bp	1.0	78.2