Hotspot

trnH-GUG-psbA

LSC · 96-607 · 512 bp

Back to Mimusops

Pi 0.00783
MarkerSeek score 47.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
512
variable_sites
4
pi
0.00783
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 TTAGTTATGGGCGAACGACG TCGTATTTCAGGCTGAGCAC 50.0 50.0 58.1 58.0 1107-1132 bp 1.0 79.6
trnH-GUG-psbA_p2 TTAGTTATGGGCGAACGACG ATTTCAGGCTGAGCACAACA 50.0 45.0 58.1 58.0 1103-1128 bp 1.0 79.6
trnH-GUG-psbA_p3 TTAGTTATGGGCGAACGACG TTCAGGCTGAGCACAACATT 50.0 45.0 58.1 58.0 1101-1126 bp 1.0 79.6
trnH-GUG-psbA_p4 TTAGTTATGGGCGAACGACG TTTCAGGCTGAGCACAACAT 50.0 45.0 58.1 58.0 1102-1127 bp 1.0 79.6
trnH-GUG-psbA_p5 TTAGTTATGGGCGAACGACG CTGATGGTATGCCCCTAGGA 50.0 55.0 58.1 58.0 1152-1177 bp 1.0 79.5