Hotspot

clpP

LSC · 26218-28257 · 2040 bp

Back to Mimosa

Pi 0.0563
MarkerSeek score 82.80
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2040
variable_sites
204
pi
0.0563
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TCCGGTAAGCGGTAAGAAGA ATACTCCAACCACCCCATGA 50.0 50.0 58.2 58.0 2731-2842 bp 1.0 89.4
clpP_p2 TCCGGTAAGCGGTAAGAAGA TGATACTCCAACCACCCCAT 50.0 50.0 58.2 58.0 2733-2844 bp 1.0 89.4
clpP_p3 TCCGGTAAGCGGTAAGAAGA AACAGGGTCAGAGGGATCAA 50.0 50.0 58.2 58.0 2645-2756 bp 1.0 89.3
clpP_p4 TCCGGTAAGCGGTAAGAAGA ACAGGGTCAGAGGGATCAAA 50.0 50.0 58.2 58.0 2644-2755 bp 1.0 89.3
clpP_p5 TCCGGTAAGCGGTAAGAAGA TCCAACCACCCCATGAATTG 50.0 50.0 58.2 58.1 2727-2838 bp 1.0 89.0