Hotspot

rps15-ycf1

SSC · 81796-82194 · 399 bp

Back to Mimosa

Pi 0.0775
MarkerSeek score 79.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
399
variable_sites
54
pi
0.0775
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAATCGATCCCGGGTTTTC GCAGCCGTTTCAGTATGGTA 50.0 50.0 58.0 58.0 523 bp 0.25 49.6
rps15-ycf1_p2 CAATCGATCCCGGGTTTTCT GCAGCCGTTTCAGTATGGTA 50.0 50.0 58.0 58.0 522 bp 0.25 49.5
rps15-ycf1_p3 TGCATTGATGGATGGTGAGG GCAGCCGTTTCAGTATGGTA 50.0 50.0 57.9 58.0 899 bp 0.25 49.4
rps15-ycf1_p4 CTTGTAGCTGGGGCATTCAT GCAGCCGTTTCAGTATGGTA 50.0 50.0 57.9 58.0 851 bp 0.25 49.3
rps15-ycf1_p5 CCCAGCCATAGTAGGTGAGA GCAGCCGTTTCAGTATGGTA 55.0 50.0 57.9 58.0 1187 bp 0.25 49.1