Hotspot

rps3-rps19

LSC · 40495-41359 · 865 bp

Back to Mimosa

Pi 0.18
MarkerSeek score 76.90
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
865
variable_sites
200
pi
0.18
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.69
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 TACCAAGTCTGAAACCGAGC AAATGATAATAGATCTCGTCGT 50.0 31.8 57.5 52.7 702-930 bp 1.0 52.0
rps3-rps19_p2 TACCAAGTCTGAAACCGAGC AATGATAATAGATCTCGTCGTT 50.0 31.8 57.5 52.7 701-929 bp 1.0 52.0
rps3-rps19_p3 GTCTGAAACCGAGCGGATTT AAATGATAATAGATCTCGTCGT 50.0 31.8 58.6 52.7 696-924 bp 1.0 52.0
rps3-rps19_p4 GTCTGAAACCGAGCGGATTT AATGATAATAGATCTCGTCGTT 50.0 31.8 58.6 52.7 695-923 bp 1.0 52.0
rps3-rps19_p5 TACCAAGTCTGAAACCGAGC AAATGATAATAGATCTCGTCGTT 50.0 30.4 57.5 53.5 702-930 bp 1.0 52.0