Hotspot

rpl36-rps8

LSC · 36060-36554 · 495 bp

Back to Mimosa

Pi 0.0688
MarkerSeek score 80.20
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
495
variable_sites
56
pi
0.0688
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 TGTCCGAATAGCGTTTCCTG GGGACGGTTCGAATACCATC 50.0 55.0 58.0 58.1 1321-1350 bp 0.75 76.6
rpl36-rps8_p2 ACTGTCCGAATAGCGTTTCC GGGACGGTTCGAATACCATC 50.0 55.0 58.0 58.1 1323-1352 bp 0.75 76.6
rpl36-rps8_p3 TGTCCGAATAGCGTTTCCTG ATGGGTAAGGACACCATTGC 50.0 50.0 58.0 57.9 1384-1413 bp 0.75 76.4
rpl36-rps8_p4 ACTGTCCGAATAGCGTTTCC ATGGGTAAGGACACCATTGC 50.0 50.0 58.0 57.9 1386-1415 bp 0.75 76.4
rpl36-rps8_p5 TTTTAGGGGGCCTACATCCA GGGACGGTTCGAATACCATC 50.0 55.0 58.0 58.1 1478-1507 bp 0.75 76.1