Hotspot

rps11-rpl36

LSC · 35793-35945 · 153 bp

Back to Mimosa

Pi 0.0556
MarkerSeek score 79.00
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
153
variable_sites
16
pi
0.0556
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 TGTCCGAATAGCGTTTCCTG AGCGGAGGGTTATAGACTCC 50.0 55.0 58.0 58.0 681 bp 0.25 49.9
rps11-rpl36_p2 ACTGTCCGAATAGCGTTTCC AGCGGAGGGTTATAGACTCC 50.0 55.0 58.0 58.0 683 bp 0.25 49.9
rps11-rpl36_p3 TTTTAGGGGGCCTACATCCA AGCGGAGGGTTATAGACTCC 50.0 55.0 58.0 58.0 838 bp 0.25 49.9
rps11-rpl36_p4 GAAATCACCTGACCCCGTAC AGCGGAGGGTTATAGACTCC 55.0 55.0 58.0 58.0 590 bp 0.25 49.8
rps11-rpl36_p5 TACGCGCATTCTTACGTGAA AGCGGAGGGTTATAGACTCC 45.0 55.0 58.0 58.0 505 bp 0.25 49.8